4D-DIA proteomics is a next-generation DIA technology based on the timsTOFPro mass spectrometer for differential quantitative proteomics analysis. 4D-DIA proteomics scanning is faster and more sensitive compared to 3D-DIA technology. Meanwhile, the addition of the fourth-dimensional ion drip separation allows DIA data to be acquired without sacrificing window cycling speed while reducing spectral complexity and improving ion utilization, achieving a comprehensive improvement in proteomics in terms of coverage depth, sensitivity, and throughput. learn more: 4d dia quantitative proteomics